Its habitats range from flat coastal plains to steep rocky canyons and hillsides; it is associated with many different vegetation types, including desert, sandy creosote areas, mesquite grassland, desert scrub, and pine-oak forests. It is common to see the western diamondback on rural blacktop roads in early evening, because of the heat retention of these surfaces, as ambient temperatures drops.
They are poor climbers. Natural predators include raptors such as hawks and eagles, roadrunners, wild hogs, and other snakes. When threatened, they usually coil and rattle to warn aggressors. They are one of the more aggressive rattlesnake species in the US in the way that they stand their ground when confronted by a foe. If rattling does not work, then the snake will strike in defense.